First Record of an Isolate (Pathotype) of Puccinia striiformis f. sp. hordei the Causative of Stripe Rust on Barley in Egypt

Document Type : Original Article

Authors

1 Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.

2 Field Crops Research Institute, Agricultural Research Center, Giza, Egypt.

Abstract

During 2018/2019 growing season, symptoms of stripe rust (yellow rust), caused by Puccinia striiformis f. sp. hordei, on barley leaves (Hordeum vulgare L.) were observed, specifically on (LIGNEE527/GERBEL/3/BOY/B*2/SURB//CI1225.2D/4/M104) and (Kv//Alger/Ceres.362/1/1/3/Arr/Esp//Alger/Ceres.362/1/1/4/CamB1//CI08887/CI05761/3/Kataja//Esp/1808/4L/5/Harmal02ArabiAbiad*2/4/Soufara02/3/R1M508/Por//WI2269Nawair1) lines, grown in El-Alameen region - Marsa Matrouh governorate (Fig., 1). Isolation of the causative was carried out from single pustules, multiplied and the obtained urediospores were used to carry out pathogenicity test on the same lines. Typical symptoms caused by Puccinia striiformis f. sp. hordei were obtained after 14 days and found to be similar to those previously collected from infected barley samples. Definition of pathogen Pathotype of P. striiformis f. sp. hordei using the molecular biology method (RAPD) DNA showed 99% similarity with Pathotype of Puccinia striiformis f. sp. hordei. On the basis of the Blast Fungi, Puccinia striiformis f. sp. hordei appeared high similarity with Pathotype as shown in (Fig., 2). This Isolate was recorded in the NCBI/gene bank under the number MN598662. This is the first record of the pathogen Pathotype of yellow rust in Egypt.

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