Determination of Patulin in Clear Apple Juice using High Performance Liquid Chromatography with Diode Array Detection

Document Type : Original Article

Authors

Central Laboratory of Residue Analysis of Pesticides & Heavy Metals in Foods (QCAP), Agricultural Research Center, 12311 Dokki, Giza, Egypt.

Abstract

Patulin is biosynthesized toxin produced by several species of filamentous fungi such as Penicillium spp., Aspergillus spp. and Byssochlamys nivea. The moulds grow on a variety of foods including
fruits and fruit products.
Patulin was recovered at different levels varied between 70-120%. The reproducibility expressed as relative standard deviation was less than 15%. The limit of quantification was 15µg/l. The method showed to be linear from the limit of quantification up to 200µg/L. The method accuracy was tested by participating in FAPAS proficiency test program. Forty-eight samples of clear apple juice collected from Egyptian local markets, half of these samples were from local production and the other imported from different countries. Patulin was detected in 22 out of 48 analyzed samples, at concentration levels ranged from 6.68 to 785.0 µg/L. The amount of patulin detected in 12 analyzed samples showed exceeding of permissible limit.

Keywords

Egyptian Journal of Phytopathology
Volume 38, Issue 1
(1-2)
2010
Pages 163-172

Files

Share

How to cite

Statistics