Molecular and functional characterization of tomatinase secreted by isolates of Fusarium oxysporum f.sp. lycopersici, the causal of vascular wilt of tomato

Document Type : Original Article

Authors

1 Institute of Plant Pathology, Agriculture Research Center, 12619, Giza Egypt.

2 Department of Plant Pathology, Faculty of Agriculture, Alexandria University

Abstract

Tomato wilt is caused by Fusarium oxysporum f. sp. lycopersici (FOL), a soilborne pathogen causing global economic losses. FOL aggressive isolates produce tomatinase to counteract tomato defense. The study aimed to identify and analyze the tomatinase gene in three FOL isolates with varying aggressiveness. Genetic analysis was employed using a polymerase chain reaction-based method, and the sequences were compared with several tomatinase sequences. Post-translational functional analysis predicted phosphorylation sites for the three tomatinase sequences using NetPhos3.1 server. Results showed that FO-19, the most aggressive isolate, had the highest tomatinase activity. Tomatinase activity was found to be correlated with phosphorylation, which may be quite important in the aggression of FOL isolates. The study highlights the importance of understanding the FOL-tomato interaction in developing effective strategies to control tomato vascular wilt.

Keywords

Main Subjects

Egyptian Journal of Phytopathology
Volume 52, Issue 2
December 2024
Pages 25-34

Files

Share

How to cite

Statistics