Assessment of Nanobriming For Soybean Against Phytophthora Root Rot: Molecular and Genetic Variation Study

Fouad, Marwa Sayed; Hassanin, Mahmoud M. H.; Eissa, Amira A. A.

doi:10.21608/ejp.2025.422950.1157

Assessment of Nanobriming For Soybean Against Phytophthora Root Rot: Molecular and Genetic Variation Study

Document Type : Original Article

Authors

¹ plant pathology research institute

² Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt.

³ Botany Department, Faculty of Agriculture, Al Azhar University.

10.21608/ejp.2025.422950.1157

Abstract

Phytophthora sojae, the causal agent of root rot on soybean was isolated and morphologically identified based on macroscopic and microscopic characters. Pathogenicity test investigated the virulence of P. sojae strain with a disease incidence of 88%. Antifungal activity of eight marjoram nano-emulsion concentrations (1000-8000ppm) was assessed against P. sojae. All concentrations caused apparent distortion in mycelial growth pattern with gradual decrease in oomycete dry biomass reaching the highest reduction ratio at 8000ppm, being 87.46%. Moreover these concentrations were screened for their potential on soybean seed germination, they exhibited a significant decrease in germination percentage with the increase in concentrations starting from 4000ppm recording 87% and the lowest ratio being (66%) at 8000 ppm. Eleven SCoT primers were selected to evaluate the alteration in genetic cellular structure in soybean seedlings treated separately with certain marjoram nano-emulsion concentrations (0, 4000, 5000, 6000, 7000 and 8000ppm). SCoT profile analysis displayed about 62 polymorphic bands with polymorphism percentage of 44.28%. Also, genome template stability (GTS%) was evaluated where it was decreased with the increase in nano-emulsion concentration ranging from 91 to 85%. In vivo application of marjoram nano-emulsion at 4000 and 7000ppm reduced disease severity significantly being 31.6±1.09% and 22.4±0.83% respectively, compared to positive control (71.2±3.42%). Growth parameters were not affected significantly, upon the treatment with nano-emulsion at 4000ppm under infection stress. But the situation was altered at 7000ppm where they were decreased significantly recording 51.5cm, 9.5 and 273.7mg for plant length, number of leaflets and plants dry biomass, respectively compared to the untreated plants.

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