Influence of Seeds Mill Extracts as Biopesticides on Meloidogyne incognita and Banana Growth

El-Ansary, Mostafa; Hamouda, Ragaa

doi:10.21608/ejp.2017.88593

Influence of Seeds Mill Extracts as Biopesticides on Meloidogyne incognita and Banana Growth

Document Type : Original Article

Authors

Mostafa El-Ansary ¹
Ragaa Hamouda ²

¹ Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

² Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

10.21608/ejp.2017.88593

Abstract

The effects of methanol extracts of some seeds mill, black seed (Nigella sativa), Jojoba (Simmondsia chinensis), Olive (Olea europaea) alone or in combination with alga, Chlorella vulgaris were tested as bio-nematicidal against root-knot nematode (RKN), Meloidogyne incognita on banana under greenhouse conditions. The total number of galls, females and egg-masses per root, also nematode soil was significantly reduced compared to untreated nematode control. Results indicated that treatment consisted of C. vulgaris + O. europaea was the most incorrodible curative for depressing the J2s numbers in soil (51.82%), than the chemical control, oxamyl (65.58%R). The phytochemical analysis of seeds mill (SMEs) and alga approved that, O. europaea had the greatest content of phenolic compound (332.14 μg gallic acid/g dry wt). This may elucidate the highly potent of the treatment, O. europaea alone or with C. vulgaris to control RKNs infestations. The maximum percentage of antioxidant activity (96.41%) was present in N. sativa. Furthermore, the greatest enhancement of growth rate was generated using alga, C. vulgaris compared with other treatments as well as untreated inoculated plants.

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