Document Type : Original Article
Authors
1
Wheat Dis. Res. Dept., Plant Pathol. Res. Inst., ARC, Giza, Egypt
2
Botany Dept., Plant Pathol. & Biotechnol. Lab., Fac. Agric., Kafr El-Sheikh Univ.
Abstract
Wheat stem rust caused by Puccinia graminis f.sp. tritici (Pgt) is one of the most destructive diseases of wheat all over the
world. In Egypt, in-spite of the absence of the new stem rust pathotype TTKS (syn. Ug99) and its variants TTKSK and TTKST, the disease takes place as a serious situation in the last few years with high disease severities on most of the common wheat cultivars during 2012-2014 growing seasons. A modified North American Pgt differential set representing the resistance genes Sr5, 21, 9e, 7b, 11, 6, 8a, 9g, 36, 9b, 30, 17, 9a, 9d, 10, Tmp, 24, 31, 38 and McN, respectively, as well as 10 supplemental Sr genes were evaluated in natural field infection. Adult resistance plants were detected on Srgenes Sr 5, Sr 9g, Sr 11, Sr 13, Sr 14, Sr 21, Sr 24, Sr 28 and Sr 38. Four specific markers were selected to detect the presence of the resistant stem rust monogenic lines including Sr13, Sr25, Sr26 and Sr31 in thirteen Egyptian bread wheat cultivars. These markers were XBE403950EST, PSY-DI, Sr26#43 STS and IAG95-STS, respectively. Stem rust resistance gene Sr26 was absent in all the tested cultivars, whereas Sr13, Sr25 and Sr31 were presented at various frequencies. The highest frequency was observed for Sr13 (100%), followed by Sr25 (84.61%) and Sr31 (46.15%). These results revealed that DNA marker for Sr26 is needed in the Egyptian cultivars and more effective genes must be identified and incorporate in adopted germplasms to face such disease.
Keywords
)
View on SCiNiTO